Regulation of the Escherichia coli HipBA Toxin-Antitoxin System by Proteolysis
Figure 5
In vivo degradation of GFP and a GFP-HipB hybrid.
GFP and GFP with C-terminal fusion to the C terminus of HipB were expressed from a pBRlacitac promoter in BW25113 (KLE907 and KLE908, respectively). The strains were grown in LB medium, and at an OD600 of 0.3 1 mM IPTG was added. After 1 h of induction, protein synthesis was inhibited by the addition of 100 µg/ml Cam, and samples for Western blots were removed over the course of 60 min. Closed squares, GFP; open squares GFP-H (GFP-HipB(73–88)). The graph represents the average of five independent experiments.