A Putative Transcription Factor MYT2 Regulates Perithecium Size in the Ascomycete Gibberella zeae
Figure 6
Total trichothecene (deoxynivalenol and 15-acetyldeoxynivalenol) production and transcriptional analyses of trichothecene synthetic genes.
(A) Each strain was grown in minimal medium supplemented with 5 mM agmatine for 7 d. Trichothecenes were analyzed by GC-MS and quantified based on the biomass produced by each strain. Asterisks indicate data that significantly differed (p<0.05) based on Tukey's test (B) Expression of Tri5 and Tri6 in the wild-type, MYT2 deletion, and MYT2 overexpression strains. Gene transcription was analyzed by quantitative real time-PCR (qRT-PCR) 4 d after inoculation in MMA. WT, G. zeae wild-type strain Z-3639; Δmyt2, MYT2 deletion mutant; MYT2com, Δmyt2-derived strain complemented with MYT2; MYT2OE, transgenic strain that has the EF1α promoter inserted in place of the MYT2 promoter region.