A Putative Transcription Factor MYT2 Regulates Perithecium Size in the Ascomycete Gibberella zeae
Figure 3
Self-fertility and asci rosettes of the G. zeae strains.
(A) Perithecia of the G. zeae strains. Five-day old carrot agar culture was mock-fertilized to induce sexual reproduction and incubated for an additional 7 d. The upper and lower panels show the photographs of perithecia formed on a whole carrot agar plate and the photographs taken with a dissecting microscope, respectively. Scale bar = 200 µm. (B) Diameter of the perithecia of the G. zeae strains. The diameters of 300 perithecia were measured for each strain using a dissecting microscope. Values with different letters are significantly different (p<0.05) based on Tukey's test. (C) Asci rosettes of wild-type and MYT2 overexpression strains. Perithecia were dissected seven days after sexual induction. Scale bar = 20 µm. WT, G. zeae wild-type strain Z-3639; myt2, MYT2 deletion mutant; MYT2com, myt2-derived strain complemented with MYT2; MYT2OE, transgenic strain that has the EF1α promoter in place of the MYT2 promoter region.