A Putative Transcription Factor MYT2 Regulates Perithecium Size in the Ascomycete Gibberella zeae
Figure 2
(A) The MYT2 promoter region was replaced with the EF1α promoter. The left and right panels show the strategy of MYT2OE strain construction and Southern hybridization, respectively. In the blot, lane 1 and lanes 2–4 represent the wild-type strain and the MYT2-overexpressed mutants, respectively. (B) Expression of MYT2 in the wild-type, MYT2 deletion, and MYT2 overexpression strains. MYT2 transcript accumulation was analyzed by quantitative real time-PCR (qRT-PCR) during the vegetative and sexual induction stages. WT, wild-type strain Z-3639; MYT2OE, transgenic strain where the MYT2 promoter region was replaced with the EF1α promoter; P, PstI. The sizes of DNA standards (kb) are indicated to the left of the blot.