NKT Cells Stimulated by Long Fatty Acyl Chain Sulfatides Significantly Reduces the Incidence of Type 1 Diabetes in Nonobese Diabetic Mice
Figure 6
Sulfatide-reactive Type II NKT cells are enriched in the PLN during the development of T1D.
PLN lymphocytes isolated from the pancreas of female NOD mice (6–20 week-old) were stained with sulfatide/CD1d-, aGalCer/CD1d- or unfilled PBS/CD1d-tetramers and anti-TCRb and then analyzed by flow cytometry. (A) The numbers shown in each panel indicate the % tetramer+ cells. (B) The scatter plots show the mean values of sulfatide/CD1d-tetramer+ (0.17%) and αGalCer/CD1d-tetramer+ (0.37%) cells in diabetic NOD mice (6–20 week-old). Note that we were not able to detect any correlation between NKT cell numbers and the severity of T1D, as we did not find any significant correlation between the frequency of sulfatide-CD1d-tetramer+ cells in diabetic vs. non-diabetic NOD mice.