NKT Cells Stimulated by Long Fatty Acyl Chain Sulfatides Significantly Reduces the Incidence of Type 1 Diabetes in Nonobese Diabetic Mice
Figure 2
C24:0 sulfatide-induced protection from the adoptive transfer of T1D is CD1d-dependent.
Female NOD.CD1d−/− mice (3–5 week-old, n = 10/group) were injected i.p. with either control vehicle (PBS) or C24:0 sulfatide (100 mg/dose) on day 0 and day 4. Pooled splenocytes and PLN lymphocytes (2×107) were transferred to female NOD.Scid recipients, and the recipient mice were monitored until 32 weeks of age for the development of T1D by determining their BGL. Two consecutive BGL readings of >250 mg/dl was considered diabetic. Data shown represent one of two representative and reproducible experiments.