Tetrahydrouridine Inhibits Cell Proliferation through Cell Cycle Regulation Regardless of Cytidine Deaminase Expression Levels
Figure 6
Tetrahydrouridine (THU) inhibits E2F1 at the protein level which is associated with the G1/S transition.
Panc-1, MIAPaCa-2, H441, and H1229 cells were treated with THU (100 µM) for 96 hrs as indicated. Subsequently, E2F1 protein levels were analyzed by western blotting. THU could down-regulate E2F1 expression. However, THU did not affect E2F1 mRNA expression level.