Tetrahydrouridine Inhibits Cell Proliferation through Cell Cycle Regulation Regardless of Cytidine Deaminase Expression Levels
Figure 5
Cytidine deaminase (CDA) inhibition does not participate in cell proliferation.
A. After transfecting siRNA against CDA, real-time PCR analysis of CDA expression in MIAPaCa-2, H441, and H1299 exhibited cell growth inhibition by THU. Statistical significance was confirmed using a t-test at Day4. B. After knocking down CDA, a proliferation assay was performed. Down-regulated CDA did not affect cell growth in MIAPaCa-2, H441, and H1229 cell lines. C. A combination therapy of CDA knockdown and THU did not show any differences compared to THU alone.