Tetrahydrouridine Inhibits Cell Proliferation through Cell Cycle Regulation Regardless of Cytidine Deaminase Expression Levels
Figure 4
THU (Tetrahydrouridine) influences the cell cycle in MIAPaCa-2, H441 and H1299.
A. After 3 days of THU exposure, the cell cycle was analyzed using flow cytometry. In accordance with cells whose growth was inhibited by THU, MIAPaCa-2, H441, and H1229 cell lines exhibited an increased rate of the G1-phase and a decrease in the S-phase. B. Cells were processed for immuno-cytochemistry with an antibody against Ki-67. Microscopic images of Ki-67-stained cells are shown in THU-treated cells and non-treated cells. Nuclei were stained with DAB. Data represents the mean ± S.D. of 3 independent experiments and significance values relate to comparisons between medium and THU treated cells.