Isocorydine Inhibits Cell Proliferation in Hepatocellular Carcinoma Cell Lines by Inducing G2/M Cell Cycle Arrest and Apoptosis
Figure 4
The relative contributions of the Chk1, Chk2 and p53 pathways.
(A) Western blot analysis of the activation of p53, MDM2, Chk1 and Chk2 in Huh7 and PLC/PFR/5 cells after ICD treatment for 48 h. (B) The cell cycle distribution of Huh7 cells after ICD treatment for 18 h following siRNA transfection. (C) Western blot analysis of the silencing effect of Chk1 or Chk2 expression levels after transfection with their corresponding siRNA. (D) Western blot analysis of p-CDK1 or CDK1 expression levels after ICD treatment for 18 h following transfection with their corresponding siRNA.