MEK1 Inhibits Cardiac PPARα Activity by Direct Interaction and Prevents Its Nuclear Localization
Figure 5
Voluntary running-wheel exercise stimulates cardiac MEK1 activation.
(a) Western blot analysis using anti-PPAR antibodies on lysates of heart samples of indicated experimental procedure, showing reduced PPAR expression of mice hearts subjected to transverse aortic constriction (TAC). (b) Quantification of PPAR protein levels of in hearts from sedentary or exercised mice (n = 6 per group). (c) Quantification of PPAR protein levels of in hearts from sham or transverse aortic constricted mice ( = 6 per group). (d) Average daily distance that mice ran voluntarily. (e) Representative images of hearts from mice that remained sedentary or were subjected to voluntary wheel exercise for 4 weeks. Note the increase in size of the exercised heart. (f) Heart weight to body weight (HW/BW) ratios of mice that remained sedentary or were subjected to voluntary wheel exercise (n = 8 per group). (g) Western blot analysis using anti-phosphorylated ERK1/2 (p-ERK1/2) antibody on lysates of indicated heart samples, demonstrating enhanced MEK1-ERK1/2 activity following exercise-induced cardiac hypertrophy. (h) Quantification of the phosphorylation status of ERK1/2 in hearts from sedentary or exercised mice (n = 6 per group).