Interference with Oligomerization and Glycosaminoglycan Binding of the Chemokine CCL5 Improves Experimental Liver Injury
Figure 5
Treatment with [44AANA47]-CCL5 leads to qualitative changes in splenocytes.
(A) Splenocytes were isolated from [44AANA47]-CCL5 and vehicle treated mice and conditioned mediate of splenocyte cultures were assessed for stellate cells migration in a Boyden chamber. Conditioned medium of splenocytes isolated from [44AANA47]-CCL5 treated mice (24 and 48 h after CCl4 application) was significantly less effective in inducing stellate cell migration compared to splenocytes isolated from vehicle treated mice. (B) Conditioned medium from mice treated [44AANA47]-CCL5 was also less effective in inducing stellate cell proliferation as assessed by BrdU incorporation. * P<0.05, ** P<0.01.