Serratamolide is a Hemolytic Factor Produced by Serratia marcescens
Figure 5
Serratamolide is hemolytic to erythrocytes and cytotoxic to epithelial cells in vitro. A.
Hemolysis of DMSO and serratamolide (1 mg/ml) to sheep red blood cells. Wells were cut into a TSA+sheep blood agar plate, and DMSO or serratamolide was added to the well and incubated for 24 hours. White arrow indicates zone of hemolysis around serratamolide treated well. B. Hemolysis of murine red blood cells in solution by serratamolide (µg/ml) incubated for 10 seconds. Distilled water was used as a complete lysis control (Lysis). A representative experiment is shown. Error bars indicate one standard deviation. C. Cytotoxicity to immortalized human airway (A549) and corneal (HCLE) epithelial cells was measured using alamar blue fluorescence that provided a positive output for viability of cells. The average of eight independent replicates is shown for each cell line from two separate experiments. Error bars indicate one standard deviation.