Anti-Inflammatory Activities of Inotilone from Phellinus linteus through the Inhibition of MMP-9, NF-κB, and MAPK Activation In Vitro and In Vivo
Figure 2
Inotilone suppresses LPS-induced MMP-9 activities (A), MMP-9 protein (B), and NF-κB expressions (C) in RAW264.7 cells.
Cells were incubated for 24 h or 1 h with 100 ng/mL of LPS in the absence or the presence of inotilone (0, 6.25, 12.5, and 25 µM). Inotilone was added 1 h before the incubation with LPS. The conditioned media were collected MMP-9 activities determined by gelatin zymography. MMP-9 activities were quantified by densitometric analysis. Representative Western blot from two separate experiments was shown. MMP-9 and NF-κB levels were calculated with reference to a LPS-stimulated culture. The data were presented as mean ± S.D. for three different experiments performed in triplicate. ###compared with sample of control group. **p<0.01 and ***p<0.001 were compared with LPS-alone group.