Polymerized Laminin-332 Matrix Supports Rapid and Tight Adhesion of Keratinocytes, Suppressing Cell Migration
Figure 9
Differential detachment of NHK cells adhered to purified Lm332 and Lm332-ECM.
NHK cells were inoculated onto the plates coated with 1 µg/ml purified Lm332 or deposited with Lm332-ECM and incubated for 1 h. After washing with PBS, the cells were incubated with trypsin/EDTA diluted 1∶35 in PBS (A) or with 10 mM EDTA alone (B). After incubation for the indicated lengths of time and then washing with PBS, the relative number of adherent cells was determined as described in Figure 5. Each bar indicates the mean ± S.D. of the fluorescent intensity (FI) for adherent cells in triplicate assays.