Polymerized Laminin-332 Matrix Supports Rapid and Tight Adhesion of Keratinocytes, Suppressing Cell Migration
Figure 2
Immunofluorescent staining of Lm332 deposited by three cell lines.
NHK (A, top), HSC-4 cells (A, center) and Lm332-HEK cells (A. bottom) were suspended in serum-free medium, inoculated at a cell density of 2×103 cells/well on collagen-coated 8-well chamber slides and incubated for 6 h. The cultures were stained for F-actin with rhodamine phalloidin (left panels) and for Lm332 with the anti-α3 chain BG5 antibody and followed by a FITC-labeled secondary antibody (center panels), as described in “Materials and Methods”. Right panels are merged images. In (B), Lm332-HEK cells were inoculated at a high cell density (1×105 cells/well), incubated for 6 h (left panel) or 48 h (right panel), and stained for Lm332 as above.