Controlled In Meso Phase Crystallization – A Method for the Structural Investigation of Membrane Proteins
Figure 4
CIMP crystallization of BR (H. salinarum).
A, Crystals from high excess water crystallization condition after 2 weeks (expected hydration level 75%). Ammonium sulfate was used as precipitant. Crystal size is approximately 11 µm. B, Crystals from excess water crystallization condition after 5 days (expected hydration level 60%). Na/K phosphate was used as precipitant. Crystal size is approximately 140 µm. C, Crystals from cubic phase crystallization conditions after 10 weeks (expected hydration level 43%). Na/K phosphate was used as precipitant. Crystal size is approximately 100 µm. D, Crystals from cubic/lamellar phase crystallization conditions after 14 weeks (expected hydration level 30%). Na/K phosphate was used as precipitant. Crystal size is approximately 200 µm.