Differential Effects of an O-GlcNAcase Inhibitor on Tau Phosphorylation
Figure 3
Analyses of major tau kinases in tau Tg mouse brains after thiamet-G treatments.
(A) Western blots of the brain homogenates developed with antibodies indicated at the left side of the blots. GAPDH blot was included as a loading control. (B) The blots were quantified densitometrically, and the immunoreactivities (mean ± SEM; n = 5) of the indicated phosphorylated kinases over that of the total kinase counterparts are shown to represent the activation statuses of these kinases. Note that the GSK-3β(Y216) antibody also recognized GSK-3α (upper band in A). Only the GSK-3β band (lower band) was used for quantification. In case of CDK and p35, GAPDH was used for normalization. *p<0.05 vs. saline injection.