Differential Effects of an O-GlcNAcase Inhibitor on Tau Phosphorylation
Figure 1
Experimental design and effects of icv injection of thiamet-G on brain protein O-GlcNAcylation.
(A) Diagram showing the experimental design of icv injection. Thiamet-G (175 µg per mouse) was injected into the lateral ventricles of the brain, and then the tau Tg mice were sacrificed 4.5 h, 9 h, or 24 h after injection. (B) The brain homogenates were analyzed by Western blots developed with monoclonal antibody RL2 against O-GlcNAcylated proteins and, as a loading control, anti-GAPDH. (C) All of the RL2-positive bands in the blots were quantified densitometrically, and the intensities of the total O-GlcNAcylated proteins after being normalized by the intensity of GAPDH are shown (mean ± SEM; n = 5; *, p<0.05; **, p<0.01; ***, p<0.001 vs. saline controls).