Rapid Determination of Myosin Heavy Chain Expression in Rat, Mouse, and Human Skeletal Muscle Using Multicolor Immunofluorescence Analysis
Figure 1
Representative images of rat red tibialis anterior (RTA) muscle showing MHC expression as well as SDH and GPD activity staining.
Panel A, rat muscle serial cross-section incubated with a primary antibody cocktail against MHCI (BA-F8), MHCIIa (SC-71), and MHCIIb (BF-F3), followed by incubation with appropriate fluorescent-conjugated secondary antibodies. Shown are type I (blue), type IIA (green), type IIB (red), type IIX (unstained), and type IIAX (intermediate green) fibers. Panel B, rat muscle serial cross-section incubated with a primary antibody cocktail against MHCIIa (SC-71) and MHCIIx (6H1), followed by incubation with appropriate fluorescent-conjugated secondary antibodies. This confirms the presence of type IIA (green) fibers, as well as confirms that the unstained fibers and intermediate green stained fibers in Panel A are type IIX (purple) and type IIAX fibers (green and purple), respectively. Panel C, rat muscle serial cross-section showing SDH activity staining. Panel D, rat muscle serial cross-section showing GPD activity staining. Bar represents 50 µm.