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Mixed Adjuvant Formulations Reveal a New Combination That Elicit Antibody Response Comparable to Freund's Adjuvants

Figure 7

50% Neutralization titer of individual pseudovirus between groups.

For single adjuvant, animals vaccinated with FCA/FIA (groups 1 and 2) have the highest titers against the tier 1A viruses, and low titers against tier 1B. Sequential immunization with different prime-boost combination of Carbopol-971P/974P and MF59 (groups 6–9) performed no better than using FCA/FIA or MF59 alone. By contrast, the combination of Carbopol-971P and MF59 (group 10) conferred significantly higher neutralization titers over all others (p<0.05), particularly against the tier 1B viruses DJ263.8 and SS1196.1.

Figure 7

doi: https://doi.org/10.1371/journal.pone.0035083.g007