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Mixed Adjuvant Formulations Reveal a New Combination That Elicit Antibody Response Comparable to Freund's Adjuvants

Figure 3

Endpoint IgG binding titer.

The antigen-specific IgG titer was measured by ELISA. Antiserum (terminal time point) was serially diluted (2-fold) and the endpoint titer was defined as the last dilution that gave a positive signal (>3-fold signal of the prebleeds). Animals that received the FCA/FIA (groups 1 and 2) or combination of Carbopol-971P and MF59 have significantly higher endpoint IgG titers than others (p<0.05).

Figure 3

doi: https://doi.org/10.1371/journal.pone.0035083.g003