Replacement of Retinyl Esters by Polyunsaturated Triacylglycerol Species in Lipid Droplets of Hepatic Stellate Cells during Activation
Figure 6
PUFA-containing TAG species, but not phospholipid species, are induced during HSC activation.
A. Contour plots of HPLC-APCI-MS analysis of HSC at day 0 and 7, showing an increase in long chain fatty acid-containing TAG species at day 7. From every ion in the m/z 880–1050 region its relative abundance (amount of blackness) and retention time in the HPLC separation is shown. TAG species with the same total number of carbon atoms in the three acyl chains (denoted on the right hand side), but different number of double bonds (:n) form diagonal “stripes” at specific m/z regions. B. Quantification of the total amount of TAG-PUFA (m/z 900–1050) and PC-PUFA species in HSCs at day 0, 4 and 7. Values are expressed relative to the level of lipid present at day 0. The results represent the means ± SEM of three experiments. C. TAG species containing one, two or three 22:5 acyl moieties are induced during HSC activation. The results represent the means ± SEM of three experiments.