Replacement of Retinyl Esters by Polyunsaturated Triacylglycerol Species in Lipid Droplets of Hepatic Stellate Cells during Activation
Figure 3
HSC activation results in a decrease in retinyl esters in HSC LDs.
Freshly isolated HSCs were cultured and fixed after 2 h (day 0; quiescent state), and day 4 and day 7 (activated state). Confocal Raman microscpectroscopy on LD enriched regions was performed as described in the Method section. Cluster image (20×20 µm2) was constructed from Raman imaging data of the square area in the white light image. Each color represents a different cluster. The cluster averages show the average Raman spectra extracted from the black, pink, green and blue clusters displayed in the cluster image. * indicates (characteristic) RE peaks; # indicates characteristic acyl peak.