Replacement of Retinyl Esters by Polyunsaturated Triacylglycerol Species in Lipid Droplets of Hepatic Stellate Cells during Activation
Figure 2
LD redistribution during HSC activation is microtubule dependent.
A. Frames from time lapse life cell analysis revealing redistribution of dynamic LDs towards growing cellular extensions. Tracking shows the typical movements of a dynamic LD from 72 h to 84 h of HSC activation. Arrows indicate other regions of dynamic LDs (trackings not shown). B. To examine microtubule involvement in the LD redistribution process, freshly isolated HSCs were after 24 h in culture either treated with 10 µM nocodazole or vehicle (control) for 72 h at 37°C and after fixation, morphology and LD localization were analyzed by differential interference contrast microscopy (DIC) and fluorescence microscopy after Bodipy staining.