Salvianolic Acid B Prevents Bone Loss in Prednisone-Treated Rats through Stimulation of Osteogenesis and Bone Marrow Angiogenesis
Figure 12
Effects of Sal B on rat marrow stromal cell (rMSC) differentiation into osteoblast.
Cells were collected from the femur marrow of one month old SD rats. Cells inoculated in 25 mm2 culture flask and cultured. The cells used in the study were the 3 passage, then transferred to the medium containing osteoblast induction medium (OB-in), Sal B and OB-in plus various concentrations of Sal B respectively. A: ALP activity (represent by OD value) was determined at 3, 5 and 7 days. B: the content of osteocalcin (µg/L) in culture medium was determined 1n day 21 after incubated with different treatment. Data shown are mean ± SD. n = 6. **P<0.01, *P<0.05 versus control (vehicle treatment).