Salvianolic Acid B Prevents Bone Loss in Prednisone-Treated Rats through Stimulation of Osteogenesis and Bone Marrow Angiogenesis
Figure 11
Effects of Sal B on the proliferation and differentiation in new born rat calvarium osteoblast.
Cells were inoculated in 96-well plates and cultured, then transferred to a medium containing various concentrations of vehicle and Sal B. MTT was tested at 24, 48, 72 and 96 h of incubation (A), ALP activity (represent by OD value) was determined later until 7 days of incubation (B), and the content of osteocalcin (µg/L) in culture medium was determined 1n day 21 after incubated with different treatment (C). Data shown are mean ± SD. n = 6. **P<0.01, *P<0.01 versus control (vehicle treatment).