Salvianolic Acid B Prevents Bone Loss in Prednisone-Treated Rats through Stimulation of Osteogenesis and Bone Marrow Angiogenesis
Figure 9
Effects of different treatments on marrow microvessel structures.
Representative micrographs of immunohistochemical staining of Von Willbrand Factor (vWF) in microvessel endothelial cells of the distal femoral metaphysis from basal (BAS), aging control (CON), Sal B alone (B40), prednisone treated (GC), GC plus Sal B40 m g/kg/d (GC+B40) and GC plus Sal B80 mg/kg/d (GC+B80). The diameter of microvessels but not density increased between 6 and 9 months (BAS v.s. CON). The GC treatment markedly decreased the density and diameter of microvessels with the appearance microvessels squeezed by the increased adipocytes (GC v.s. BAS & CON). Sal B treatment prevented the GC induced changes. High dose (Sal B80 mg/kg/d) treatment significant increased the diameter of the microvessels (double arrows) (vs. GC & CON). Quantitative measurements of microvessel diameter (DMV) and density (MVD) are shown in table 8.