Salvianolic Acid B Prevents Bone Loss in Prednisone-Treated Rats through Stimulation of Osteogenesis and Bone Marrow Angiogenesis
Figure 8
Effects of different treatments on osteoblast morphology and adipocyte distribution and corresponding PPARγ expression.
Representative micrographs in basal (BAS), vehicle (aging) control (CON), prednisone (GC) and GC plus 80 mg Sal B/kg/d (GC+B80) treated cancellous bone in distal femoral metaphysic. Arrows point to osteoblasts (Ob, Goldner's Trichrome stain). Active osteoblasts are present as multi- plump columnar lining on the trabecular surface in BAS and CON rats. The GC treatment induced the appearance of shriveling and inactive osteoblasts (v.s. BAS & CON) while the Sal B treatment protected GC-induced osteoblast impairment (GC+B80 v.s.GC). Adipocyte content (F.Ar, Hematoxylin stain) and corresponding immunohistochemical staining of Peroxisome Proliferator-Activated Receptor γ (PPARγ) expression (arrows from spots, PPARγ stain) increased between 6 and 9 months (BAS v.s. CON). The GC treatment markedly increased adipocyte number and size (GC v.s. BAS & CON), and the amount of PPARγ positive cells, while the Sal B treatment prevented the GC-induced increases (GC+B80 v.s.GC). Quantitative measurements of osteoblasts, fatty area and PPARγ expression are shown in table 6 and table 8.