In Vitro Studies on Erythrosine-Based Photodynamic Therapy of Malignant and Pre-Malignant Oral Epithelial Cells
Figure 7
Confocal Laser Scanning Microscopy (CLSM) Analysis.
CLSM images of DOK (A) and H357 (B) cells at different recovery time points post-PDT (2, 4 and 24 h) were taken for both low (71.03 µM) and high (1136.50 µM) doses of erythrosine. For both doses, PDT irradiation was done with a fluence of 122.58 J/cm2. Green (SYTO9) fluorescence represents both live and dead cells while red (Propidium Iodide) fluorescence stains the dead cells alone. The arrow in the DOK cells (A) indicates the presence of apoptotic bodies whereas the arrow in the H357 cells (B) points to a group of necrotic cells.