In Vitro Studies on Erythrosine-Based Photodynamic Therapy of Malignant and Pre-Malignant Oral Epithelial Cells
Figure 3
Erythrosine co-localization analysis.
Sub-cellular co-localization of erythrosine in both DOK and H357 cells. (A and B) LysoTracker localization (for lysosomal visualization) and MitoTracker localization (for mitochondrial visualization) was compared with 1136.50 µM erythrosine localization in both DOK (A) as well as H357 (B) cells. Also, co-localization frequency scatter plots, Pearson's coefficient (r) and Mander's coefficient (M) were calculated to evaluate the overlap between LysoTracker/MitoTracker localizations and erythrosine localizations. (C) Co-localization frequency colour map indicates the estimation of co-localizations on the basis of ‘heat map schemes’ such that hotter colours tend to indicate high co-localization. Thus, red/maroon indicates 100% co-localization, yellow-green indicates 50% co-localization and purple/dark blue indicates 0% co-localization.