In Vitro Studies on Erythrosine-Based Photodynamic Therapy of Malignant and Pre-Malignant Oral Epithelial Cells
Figure 2
The pre-malignant (DOK) and malignant (H357) oral epithelial cells were incubated with different concentrations of erythrosine B (71.03 µM, 142.06 µM, 284.13 µM, 568.27 µM and 1136.50 µM) followed by PDT irradiation with different fluences (0 J/cm2, 40.86 J/cm2, 81.72 J/cm2 and 122.58 J/cm2). Following PDT, the cells were recovered 24 h later and percentage (%) of cell killing caused by PDT was estimated. (A) DOK cell's susceptibility to erythrosine-based PDT and (B) H357 cell's susceptibility to erythrosine-based PDT. The values are means of six replicate determinations ± S.D. Statistical analysis was done via student's t-test with significance set at P<0.05 (* indicates statistical comparison of a data-point within one erythrosine B concentration group vs. the corresponding data values of 0 J/cm2 fluencies for that group).