Cysteamine Suppresses Invasion, Metastasis and Prolongs Survival by Inhibiting Matrix Metalloproteinases in a Mouse Model of Human Pancreatic Cancer
Figure 8
Cysteamine decreased MMP activity in primary orthotopic tumors in mice.
A. At day 30 after orthotopic implantation of pancreatic cancer cells, tumors were collected, total protein extracted and MMP activity determined by using fluorogenic MMP substrate. B. MMP-9 mRNA levels were determined in total RNA extracted from tumors by qRT-PCR. β-actin was used for a reference gene. C. MMP-9 protein level was determined by ELISA. D. Zymographic assay was performed in cysteamine treated tumors. Data are expressed as mean ± S.D. of triplicate to quadruplicate determinations. Experiment was repeated two times. Statistical significances are shown by *: P<0.05, †: p<0.01 and ‡: P<0.001.