Cysteamine Suppresses Invasion, Metastasis and Prolongs Survival by Inhibiting Matrix Metalloproteinases in a Mouse Model of Human Pancreatic Cancer
Figure 5
Cysteamine decreased metastasis and prolonged survival in an orthotopic pancreatic cancer mouse model.
HS766T and MIA-PaCa2 cells (2×106) were implanted directly into the pancreas of 5–6 week-old female nude nu/nu mice and then peritoneal cavity and skin were closed using clips. From day 4 after tumor implantation, mice were treated twice a day with vehicle or 25, 100, 250 mg/kg/day of cysteamine until the end of the experiment. The mice with tumors were sacrificed 4 weeks after cell implantation. A. A representative mouse from each treatment group harboring MIA-PaCa2 tumor is shown. Yellow arrows indicate primary tumors in the pancreas and blue triangles indicate metastatic lesions. In another experiment, control and cysteamine treated mice were followed for survival. Kaplan-Meier survival curves of mice harboring HS766T (B) and MIA-PaCa2 (C) tumors.