Unlike for Human Monocytes after LPS Activation, Release of TNF-α by THP-1 Cells Is Produced by a TACE Catalytically Different from Constitutive TACE
Figure 4
Immunofluorescence of membrane-bound TNF-α on the surface of THP-1 cells and human monocytes after Ro 32-7315 treatment.
Cells were stimulated for 4 hours with LPS (1 µg/ml) in the presence of Ro 32-7315. Then, cells were stained by FITC-anti-m-TNF-α MAb. Image acquisition was made on a confocal microscope. The numerical recording of the images and analysis were carried out in Image J (Wayne Rasband, National Institute of Mental Health, Bethesda, Maryland, USA.).