Unlike for Human Monocytes after LPS Activation, Release of TNF-α by THP-1 Cells Is Produced by a TACE Catalytically Different from Constitutive TACE
Figure 3
PBMC surface accumulation of the membrane-bound TNF-α after Ro 32-7315, Celastrol and TQ treatment.
Cells were stimulated for 24 hours with 5 µg/ml of LPS in the presence of 10 µM of Ro 32-7315, TQ and Celastrol. 0.1% (v/v) DMSO was used as a control (silver histogram). The non-permeabilized cells were then stained with FITC-anti-m-TNF-α MAb. Membrane TNF-α expression was evaluated by flow cytometry. The representative histograms are shown. The fluorescence intensity (AU) is plotted against the number of cells (y-axis). For all 3 compounds, membrane–bound TNF-α expression increased after THP-1 cells were treated (p<0.05, n = 3).