Unlike for Human Monocytes after LPS Activation, Release of TNF-α by THP-1 Cells Is Produced by a TACE Catalytically Different from Constitutive TACE
Figure 1
Inhibition of TNF-α secretion by different human cell types in presence of Ro 32-7315, TQ and Celastrol.
Cells (5×105/ml) were incubated for 4 hours (for human monocytes and THP-1 cells) or 24 hours (for PBMC) with 1or 5 µg/ml LPS respectively, in the presence of ranging compounds concentrations or 0.1% (v/v) DMSO as a control. The TNF-α levels were then assessed in culture supernatant by ELISA technique. Dose-response curves were fitted on GraphPad PRISM (v.4.0, GraphPad Software, Inc. La Jolla, CA,USA) sigmoidal dose-response curve-fit model (n = 3 independent experiments); s-TNF-α correspond to the secreted form of TNF-α. Statistical difference between IC50 of Ro 32-7315, TQ and Celastrol are indicated in Table 1.