Molecular Characterisation of Transport Mechanisms at the Developing Mouse Blood–CSF Interface: A Transcriptome Approach
Figure 5
Cellular and subcellular distribution of GYPA immunoreactivity in E15 and adult choroid plexus.
A. Embryonic day 15 (E15) lateral ventricular choroid plexus. The more medial younger segment of the plexus emerging from the hem and hippocampal anlage is shown in the lower and right side of the figure. This part of the plexus shows only weak immunopositive staining for GYPA (filled arrowheads). At the tip of the plexus most epithelial cells exhibit a strong granular immunoreactivity in the apical cytoplasm (arrows) and an apical membrane-associated surface immunostaining (open arrowheads). Basolateral cell membranes (BM) are also immunopositive, as are vascular endothelial cells, which show a marked immunoreactivity (open arrows), in particular in areas with basolateral and apical epithelial membrane-associated immunostaining. Strong membrane immunostaining of erythrocytes (e) is also evident. B. Adult lateral ventricular choroid plexus. A very weak fine granular cytoplasmic immunoreactivity is seen in almost all choroid plexus epithelial cells in contrast to a stronger immunoreactivity found in few small segments of the plexus (framed area). Note the apparent lack of cytoplasmic immunostaining in the apical-most part of most epithelial cells (filled arrowheads), but a distinct immunostaining of small vesicles within the cytoplasm (arrows). Very few cells exhibit an apical membrane-associated immunoreactivity (open arrowheads). There was no immunoreactivity for GYPA in vascular endothelial cell membranes (open arrows). Scale bar: 10 µm in all.