A Novel Intravital Method to Evaluate Cerebral Vasospasm in Rat Models of Subarachnoid Hemorrhage: A Study with Synchrotron Radiation Angiography
Figure 5
Photomicrographs (A) show HE staining of ACA (a, b, c) and MCA (d, e, f) following SAH in control (a, e), cisterna magna blood injection (b, e) and prechiasmatic cistern blood injection groups (c, f). Scale bar = 50 µm.
Macroscopic images of brain samples after saline perfusion and paraformaldehyde fixation (B). Brains are obtained from G1 (a), G2 (b), G3 (c) and G4 (d) groups, respectively. Arrows indicate the blood clot at the basal subarachnoid space. Scale bar = 2 cm. Bar graphs (C and D) summarize the MCA and ACA diameter in G1, G2, G3, and G4 groups at each time point after operation. Data are means±SD. n = 5 in each group. *, p<0.05, G1 vs. G3, G2 vs. G4. Three dimensional photomicrographs (E) show major cerebral arteries of the Circle of Willis in control (a) and SAH rats (b) using micro-XCT imaging technique.