Granzyme B Cleaves Decorin, Biglycan and Soluble Betaglycan, Releasing Active Transforming Growth Factor-β1
Figure 3
GrB cleaves native smooth muscle cell-derived decorin and biglycan.
HCASMCs were incubated at confluency for adequate ECM synthesis. Cells were removed, GrB was incubated with the ECM and decorin and biglycan cleavage fragments were detected by western immunoblotting.