Monodansylpentane as a Blue-Fluorescent Lipid-Droplet Marker for Multi-Color Live-Cell Imaging
Figure 5
LD trafficking monitored in living cells using MDH.
HeLa cells stained with MDH (A, left) were imaged every 2 seconds for 20 frames. Single LD trajectories (A, right, showing the movement of the LD in the white dotted rectangle) were then individually analyzed. LDs stained by MDH (B, top) showed similar active transport speeds compared to those stained with BODIPY 493/503 (B, bottom) (150 velocities each, in HeLa cells). The two distributions displayed similar mean (0.11 µm/s vs. 0.12) and standard deviation (0.09 vs. 0.11).