Monodansylpentane as a Blue-Fluorescent Lipid-Droplet Marker for Multi-Color Live-Cell Imaging
Figure 2
A comparison between the spectral properties of commercially available LD dyes and fluorescent proteins.
The absorbance (A) and emission spectra (B) of LD dyes in sunflower seed oil, overlaid with fluorescent proteins' respective curves in water (the TagGFP2, TagYFP, and TagRFP spectra were obtained from Evrogen). The absorbance spectrum of MDH is significantly blue-shifted compared to those of the green and red fluorescent proteins. The 420–480 nm emission from MDH in oil is also free of overlap from the green and red fluorophores.