Nef Alleles from All Major HIV-1 Clades Activate Src-Family Kinases and Enhance HIV-1 Replication in an Inhibitor-Sensitive Manner
Figure 9
Inhibition of HIV-1 Nef chimeras by 4-amino DFP analogs.
U87MG-CD4+/CXCR4+ cells (upper panel) and CEM-T4 cells (lower panel) were infected with wild-type HIV-1 NL4-3, a Nef-defective mutant (ΔNef), or the indicated Nef chimeras as described in the legends to Figures 7 and 8. DFP-4A, DFP-4AP, or DFP-4AB were added to the cultures to a final concentration of 3 µM, and viral replication was determined by p24 ELISA 5 days (U87MG) or 10 days (CEM-T4) later. Data are expressed as the mean percent of HIV-1 replication observed in control cultures incubated with the carrier solvent (0.1% DMSO) ± S.D. (n = 6).