A Pivotal Role of Vitamin B9 in the Maintenance of Regulatory T Cells In Vitro and In Vivo
Figure 4
Vitamin B9 is IL-2-independent survival factor for Treg cells.
(A) The amounts of intracellular vitamin B9 were measured using purified CD4+FR4hi Treg or CD4+FR4low/− non-Treg cells. Data are means ± SEM (n = 4). (B, C) Experiments similar to those shown in Fig. 1B were performed in the presence of anti-CD3 antibody stimulation with or without IL-2 stimulation. Cell number of Foxp3+CD4+ T cells (B) and the expression of phosphorylated STAT5 (pSTAT5) in Foxp3+CD4+ T cells (C) were determined. Data in (B) are means ± SEM (n = 6). Similar results were obtained from 3 separate experiments.