A Novel Cold-Regulated Cold Shock Domain Containing Protein from Scallop Chlamys farreri with Nucleic Acid-Binding Activity
Figure 7
Analysis of nuclear acids binding activity of CfCSP by gel shift assay.
The purified rCfCSP proteins were incubated with ssDNA (A), M13mp8 dsDNA RFI DNA (dsDNA) (B) and the in vitro transcribed luciferase mRNA (C) to analyze the effect of CfCSP on the formation of nucleotide-protein complexes. A range of CfCSP fusion proteins from 0 to 300 pmol were used for analysis. Arrow shows nucleotide-protein complex.