Trypacidin, a Spore-Borne Toxin from Aspergillus fumigatus, Is Cytotoxic to Lung Cells
Figure 10
Flow cytometry analysis of the cell cycle.
A549 and HBEpC were exposed to 1, 10, 30 and 50 µM trypacidin for 24 h in culture conditions. In the negative control (0 µM trypacidin), cells were exposed to 1% DMSO. Gliotoxin 5 µM was used as positive control to induce sub-G1 particles. The cells were then harvested and analysed for cell cycle as described in the Materials and Methods. The figure shows mean values for 3 independent experiments.