An Osteoblast-Derived Proteinase Controls Tumor Cell Survival via TGF-beta Activation in the Bone Microenvironment
Figure 5
Absence of host MMP-2 does not impair osteoclast precursor function.
A, Quantitative analysis of isolated wild type (WT) and MMP-2 null (MMP-2−/−) CD11b+ve osteoclast precursor migration to 10% serum or control serum free media. B, Analysis of the osteoclastogenic capability of WT or MMP-2−/− osteoclast precursors. Arrows indicate TRAcP positive (red) multinucleated (blue) osteoclasts. Scale bars represent 50 µm. C, Analysis of the number of WT and MMP-2−/− mature osteoclasts formed/well in each group (n≥3/group). D, Pit formation assay on dentin discs to test the functionality of mature WT and MMP-2−/− osteoclasts. Asterisk denotes that p<0.05 while p = n.s. denotes non-significant p values.