Role of Palladin Phosphorylation by Extracellular Signal-Regulated Kinase in Cell Migration
Figure 5
ERK phosphorylates palladin in an in vitro kinase assay.
GST-fused residues 45–249 of wild-type or mutant palladin was incubated with active ERK for 10 min at 37°C in the presence of radioactive [γ32−P]-ATP and separated by SDS-PAGE. Left panel shows the phosphorylation of recombinant proteins, and the right panel shows Coomassie blue staining of recombinant proteins.