GW8510 Increases Insulin Expression in Pancreatic Alpha Cells through Activation of p53 Transcriptional Activity
Figure 1
Effects of GW8510 treatment on mouse alpha cells and dissociated human islet cells.
Pancreatic gene expression was measured by quantitative real-time RT-PCR (qPCR) following a (A) 5-day dose-response and (B) time-course with 1.65 µM GW8510. (C) Insulin secretion measurements in dissociated human islets following 5-day compound treatment at indicated concentrations. (D) Immunofluorescence analysis quantification of total cell numbers, measured by nuclear count, and numbers of alpha and beta cells, measured by glucagon and insulin staining, respectively, following compound treatment. (E) Representative images shown. All data represent the mean±SD of at least three experiments; *p<0.05, **p<0.01 and ***p<0.001.