Efficient Conversion of Astrocytes to Functional Midbrain Dopaminergic Neurons Using a Single Polycistronic Vector
Figure 3
Transcriptional profile of astrocyte-derived dopaminergic neurons.
Heat map of quantitative RT-PCR results comparing astrocyte-derived neurons magnetically sorted for a MAP2-CD4 reporter. Ast, uninfected astrocytes. ESN, mouse embryonic stem cell-derived neurons, generated via co-culture with PA6 stromal cells. Induced dopaminergic neurons express markers consistent with midbrain dopaminergic neurons. Color scale indicates change in Ct (threshold cycle) relative to the normalizing actin control. Higher delta Ct values correspond to lower relative gene expression, with every Ct decrease of 3.3 representing a ten-fold increase in relative expression.